Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Simple Doublet Lens Design for Mid-Infrared Imaging.

Applied spectroscopy·2024

Same author

β-Bracelets: Macrocyclic Cross-β Epitope Mimics Based on a Tau Conformational Strain.

Journal of the American Chemical Society·2023

Same author

Cross-α/β polymorphism of PSMα3 fibrils.

Proceedings of the National Academy of Sciences of the United States of America·2022

Same author

A theoretical analysis of coherent cross-peaks in polarization selective 2DIR for detection of cross-α fibrils.

The Journal of chemical physics·2022

Same author

Local Electric Fields in Aqueous Electrolytes.

The journal of physical chemistry. B·2021

Same author

Does liquid-liquid phase separation drive peptide folding?

Chemical science·2021

Same journal

On the Nonparametric Diabatization of Coupled Electronic States.

The journal of physical chemistry. A·2026

Same journal

Stability of Some Ternary 13-Atom Icosahedral Clusters Assessed with Geometric, Electronic, and Thermodynamic Criteria.

The journal of physical chemistry. A·2026

Same journal

A Three-Phase Distribution Method for Quantifying the Intermolecular Interactions.

The journal of physical chemistry. A·2026

Same journal

Cooperative Effects in the Inverse Coordination Complexes of Aromatic Azines and Tin(IV) Halides.

The journal of physical chemistry. A·2026

Same journal

The Infrared Spectra of Neutral Dimethyl-Sulfide, -Disulfide and -Sulfoxide Biomarkers in Molecular Beams.

The journal of physical chemistry. A·2026

Same journal

Photoinduced Charge-Transfer Suppresses Triplet Formation Efficiency in Thiocoumarins: Evidence from Ultrafast Spectroscopy and Theoretical Calculations.

The journal of physical chemistry. A·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 18, 2025

Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors

Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors

Published on: January 29, 2013

Resolution Enhancement in Wide-Field IR Imaging and Time-Domain Spectroscopy Using Dielectric Microspheres.

Dean N Edun¹, Claire E Nelmark¹, Arnaldo L Serrano¹

¹Department of Chemistry and Biochemistry, University of Notre Dame, 251 Nieuwland Science Hall, Notre Dame, Indiana 46544, United States.

The Journal of Physical Chemistry. A

|June 17, 2020

Summary

This summary is machine-generated.

Dielectric microspheres enhance infrared microscope resolution for super-resolution imaging. This breakthrough enables advanced techniques like ultrafast infrared imaging, paving the way for new scientific discoveries.

More Related Videos

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Related Experiment Videos

Last Updated: Dec 18, 2025

Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors

Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors

Published on: January 29, 2013

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

Published on: March 22, 2019

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Area of Science:

Optics and Photonics
Microscopy
Spectroscopy

Background:

Wide-field imaging with dielectric microspheres offers super-resolution at visible wavelengths.
Current limitations exist in extending this technique to infrared (IR) imaging for enhanced resolution.

Purpose of the Study:

To demonstrate the use of dielectric microspheres for enhancing far-field resolution in wide-field infrared microscopy.
To explore the feasibility of spectroscopic imaging through microspheres using advanced laser systems.

Main Methods:

Utilized polystyrene microspheres in a wide-field infrared microscope setup.
Employed a pulse-shaper based femtosecond mid-IR laser system for spectroscopic imaging.

Main Results:

Achieved substantial improvements in resolution and magnification using dielectric microspheres.
Successfully demonstrated spectroscopic imaging capabilities through the microspheres in the mid-IR range.

Conclusions:

Dielectric microspheres are effective for enhancing resolution in infrared microscopy.
This technique shows promise for ultrafast IR imaging methods like pump-probe and 2DIR microscopy.