Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

969
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
969
Antibiotic Selection00:57

Antibiotic Selection

59.1K
Overview
59.1K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.5K
Transduction01:16

Transduction

948
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
948
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

47.8K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
47.8K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

386
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
386

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short autoinhibitory sequences control phase separation of an essential bacterial transcription termination factor.

The EMBO journal·2026
Same author

A conserved peptidase governs glucose homeostasis in <i>Bacteroides</i>.

bioRxiv : the preprint server for biology·2026
Same author

The master virulence regulator PhoP dictates carbon metabolism by controlling cyclic AMP synthesis in Salmonella.

PLoS biology·2025
Same author

How Pathogens Maintain Proteostasis During Infection.

Molecular microbiology·2025
Same author

Hierarchical glycolytic pathways control the carbohydrate utilization regulator in human gut Bacteroides.

Nature communications·2025
Same author

Infection-relevant conditions dictate differential versus coordinate expression of <i>Salmonella</i> chaperones and cochaperones.

mBio·2025

Related Experiment Video

Updated: Dec 18, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

12.2K

A Physiological Basis for Nonheritable Antibiotic Resistance.

Mauricio H Pontes1,2, Eduardo A Groisman3,4

  • 1Department of Pathology and Laboratory of Medicine, Penn State College of Medicine, Hershey, Pennsylvania, USA mpontes@pennstatehealth.psu.edu eduardo.groisman@yale.edu.

Mbio
|June 18, 2020
PubMed
Summary

Bacterial antibiotic tolerance, including persister cells, can arise from nonheritable mechanisms. Nutrient limitation or slowed growth conditions promote this tolerance by inhibiting core cellular processes.

Keywords:
antibiotic tolerancegrowth feedback regulationpersister

More Related Videos

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

1.4K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.5K

Related Experiment Videos

Last Updated: Dec 18, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

12.2K
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

1.4K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.5K

Area of Science:

  • Microbiology
  • Molecular Biology

Background:

  • Antibiotics are crucial in modern medicine but face challenges from bacterial survival.
  • Bacterial survival can stem from genetic changes or nonheritable antibiotic tolerance.
  • Nonheritable tolerance includes persister cells, a small subpopulation surviving antibiotic exposure.

Purpose of the Study:

  • To investigate the underlying mechanisms of nonheritable antibiotic tolerance in bacteria.
  • To determine the role of cellular processes in the development of antibiotic persistence.

Main Methods:

  • The study reviews existing literature on bacterial antibiotic tolerance and persistence.
  • It analyzes the impact of nutrient limitation, bacteriostatic antibiotics, and growth-retarding gene expression.

Main Results:

  • Nonheritable antibiotic resistance is not solely dependent on specific molecules like toxin-antitoxin systems, ATP, or guanosine polyphosphates.
  • Conditions such as nutrient limitation and slowed bacterial growth consistently enhance nonheritable resistance.
  • These conditions promote feedback inhibition within core cellular processes.

Conclusions:

  • Antibiotic persistence is proposed to result from feedback inhibition in core cellular processes.
  • This inhibition leads to a phenotypic slowdown or cessation of bacterial growth, conferring tolerance.