Related Experiment Video
Updated: Dec 18, 2025
10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
36.3K
TOP2A Promotes Cell Migration, Invasion and Epithelial-Mesenchymal Transition in Cervical Cancer via Activating the
Bi Wang1,2, Yaping Shen3, Yin Zou4
1Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou, People's Republic of China.
Cancer Management and Research
|June 18, 2020
Summary
Topoisomerase IIA (TOP2A) is highly expressed in cervical cancer, promoting cell migration, invasion, and epithelial-mesenchymal transition (EMT) by activating the PI3K/AKT pathway.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Topoisomerase IIA (TOP2A) exhibits high expression in cervical cancer.
- The specific role of TOP2A in cervical cancer progression, particularly in migration, invasion, and EMT, is not well understood.
Purpose of the Study:
- To investigate the effect of TOP2A on cervical cancer cell migration, invasion, and epithelial-mesenchymal transition (EMT).
- To elucidate the underlying molecular mechanism by which TOP2A influences these processes, focusing on the PI3K/AKT signaling pathway.
Main Methods:
- Western blotting to assess TOP2A expression in cervical cancer tissues and cell lines.
- Transwell assays to evaluate cell migration and invasion capabilities.
- Analysis of cell morphology and epithelial-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin).
- Inhibition of PI3K/AKT signaling using LY294002 to determine its role in TOP2A-mediated effects.
Main Results:
- TOP2A was overexpressed in 85% of cervical cancer tissues compared to normal tissues.
- TOP2A repression in SiHa cells reduced migration, invasion, and induced an epithelial phenotype (increased E-cadherin).
- TOP2A overexpression in Hela cells enhanced migration, invasion, and EMT (decreased E-cadherin, increased N-cadherin).
- TOP2A modulates PI3K/AKT signaling, with increased p-AKT upon TOP2A overexpression and decreased p-AKT upon TOP2A silencing.
- Inhibition of PI3K/AKT signaling partially reversed TOP2A-induced promotion of migration, invasion, and EMT.
Conclusions:
- TOP2A is abnormally overexpressed in cervical cancer.
- TOP2A overexpression drives cervical cancer cell migration, invasion, and EMT.
- These effects are mediated through the activation of the PI3K/AKT signaling pathway.
Keywords:
PI3K/AKT signalingcell morphologyepithelial–mesenchymal transitionmigrationtopoisomerases type IIAMore Related Videos
06:54Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
14.0K
07:47Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
Published on: September 15, 2023
2.0K
Related Concept Videos
01:35Cancer Cell Migration through Invadopodia
3.1K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.1K
03:03mTOR Signaling and Cancer Progression
4.5K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.5K
01:22PI3K/mTOR/AKT Signaling Pathway
5.1K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.1K
02:30Metastasis
6.3K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.3K
01:18Role of Myosin in Cell Migration
3.0K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.0K
01:21Cell Polarization by Rho Proteins
3.4K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.4K