Sensing the classical swine fever virus with molecularly imprinted polymer on quartz crystal microbalance

Classical swine fever (CSF) is a highly contagious and fatal viral disease in pigs caused by the virus of the same name (classical swine fever virus - CSFV). Economical reasons dictate the need for rapid early detection of this pathogen. Herein we report on a sensor for CSFV detection based on a quartz crystal microbalance (QCM) making use of molecularly imprinted polymer (MIP) as the receptor. It relies on a copolymer comprising acrylamide (AAM), methacrylic acid (MAA), methyl methacrylate (MMA), and n-vinylpyrrolidone (VP). SEM images of CSFV MIP reveal cavities on the polymer surface with an average diameter of d = 59 nm, which correlates well with the dimensions of CSFV particles. QCM sensor measurements yield concentration-dependent CSFV sensor responses resulting in LOD = 1.7 μg/mL, LOQ = 5.1 μg/mL and R² = 0.9963. Furthermore, CSFV-MIP sensors selectively bind CSFV with selectivity factors of 2 over porcine respiratory and reproductive virus (PRRSV) and 62 over pseudorabies virus (PRV), respectively. Finally, sensor responses turned out fully reversible.