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Updated: Dec 18, 2025

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Published on: April 1, 2014
Advances in various techniques for isolation and purification of sterols
Sneha Dikshit1, Sakshi Bubna1, Anand Gupta1
1Amity Institute of Biotechnology, Amity University Mumbai, Bhatan, Post-Somatane, Panvel, Navi Mumbai, Maharashtra 410206 India.
Abstract:
Plants consist of triterpenoids such as phytosterols (PT) (C29H50O) with steroidal nuclei, including sitosterol, stigmasterol, brassicasterol and campesterol. They are hydrophobic but soluble in alcohol and other organic solvents and are isolated from industrial waste deodorizer distillates of various edible oil industries. They exist as free PT or their ester derivatives in soybean, rice, wheat, oat, cottonseed and corn fiber, and other cereals and grains. Conventional isolation techniques such as solvent extraction, distillation, evaporative fractionation, saponification and chemical esterification are employed for isolation and purification of PT. The present article reviews the various advanced separation techniques like solvent crystallization, supercritical fluid extraction, high speed counter-current chromatography and enzymatic process as strategic methods to isolate and purify sterols.
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