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Methylglyoxal and soluble RAGE in type 2 diabetes mellitus: Association with oxidative stress
Alisha Reyaz1, Sana Alam1, Kailash Chandra1
1Department of Biochemistry, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, 110062 New Delhi, India.
Purpose:
Methylglyoxal (MGO) and MGO related advance end product (AGE) are thought to contribute to the development of diabetes and its complications. The present study was intended to determine plasma MGO and sRAGE levels in T2DM patients and to assess the relationship between MGO and other parameters, such as sRAGE and oxidative markers.
Methods:
The study was carried out in 100 control and T2DM subjects. Methylglyoxal, sRAGE, HbA1c, and other markers were measured by using a standard protocol and the relationship between variables was analyzed using Spearman's correlation analysis.
Results:
Plasma MGO levels in patients with T2DM (221.1 ± 9.50 ng/mL) were significantly higher than in control subjects (121.1 ± 6.52 ng/mL, P < 0.001). The plasma level of MGO was positively correlated with glycosylated hemoglobin (HbA1c, r = 0.50, P < 0.001). Plasma soluble form of RAGE (sRAGE) was significantly decreased in T2DM subjects (5.3 ± 0.64 ng/mL) as compared to the control group (7.7 ± 0.86 ng/mL, p < 0.05). However, at increased level of glycation (HbA1c > 10%), the sRAGE level was 6.2 ± 0.42 ng/mL and was not statistically significant as compared to control healthy group (> 0.05). Moreover, we have not found any correlation between MGO and other markers (p > 0.05).
Conclusions:
The findings of the present study showed that increased plasma MGO level is significantly associated with the HbA1c levels in T2DM patients. Moreover, the study shows that plasma sRAGE level is significantly augmented at increased level of glycation (HbA1c > 10%) in T2DM patients.
Insights
Elevated methylglyoxal (MGO) is linked to type 2 diabetes (T2DM). While MGO correlates with HbA1c, soluble RAGE (sRAGE) levels decrease in T2DM, but increase with higher glycation.
Area of Science:
- Biochemistry
- Endocrinology
- Metabolic Diseases
Background:
- Methylglyoxal (MGO) and its advanced glycation end products (AGEs) are implicated in diabetes pathogenesis.
- Understanding the roles of MGO and soluble RAGE (sRAGE) is crucial for managing type 2 diabetes (T2DM) and its complications.
Purpose of the Study:
- To quantify plasma MGO and sRAGE levels in T2DM patients.
- To investigate the correlation between MGO, sRAGE, and other metabolic markers, including HbA1c and oxidative stress indicators.
Main Methods:
- A cohort of 100 control and T2DM subjects was analyzed.
- Plasma levels of MGO, sRAGE, and HbA1c were measured using standard assays.
- Spearman's correlation analysis was employed to assess relationships between variables.
Main Results:
- T2DM patients exhibited significantly higher plasma MGO levels compared to controls (221.1 vs. 121.1 ng/mL).
- Plasma MGO positively correlated with HbA1c (r=0.50, P<0.001).
- Plasma sRAGE levels were significantly lower in T2DM patients (5.3 vs. 7.7 ng/mL), but increased at HbA1c >10%.
Conclusions:
- Increased plasma MGO is significantly associated with elevated HbA1c in T2DM.
- Plasma sRAGE levels appear to be modulated by glycation levels in T2DM patients.
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