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Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Updated: Dec 18, 2025

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

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Published on: January 11, 2011

Efficient FLP-mediated germ-line recombination in C. elegans

Javier Macías-León¹, Peter Askjaer¹

¹Andalusian Center for Developmental Biology (CABD), CSIC/JA/Universidad Pablo de Olavide, 41013 Seville, Spain.

Micropublication Biology

|June 19, 2020

Summary

No abstract available in PubMed .

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