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Related Concept Videos

In-vitro Mutagenesis01:16

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Related Experiment Video

Updated: Dec 18, 2025

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
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Mouse Ptchd3 is a non-essential gene.

Shaimar R González Morales1, Chengjing Liu1, Heath Blankenship1

  • 1Department of Biology, Marshall University, Huntington WV25755, USA.

Gene: X
|June 19, 2020
PubMed
Summary

The patched domain containing 3 (Ptchd3) gene is not essential for mouse development or sperm production. Knockout mice lacking Ptchd3 showed normal growth, fertility, and spermatogenesis, indicating no significant role in these processes.

Keywords:
Dhh, desert hedgehogHedgehog signalingHh, hedgehogKnockoutMale germ cellsPtchd3Ptchd3, patched domain containing 3Spermatogenesis

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Molecular genetics

Background:

  • The patched domain containing 3 (Ptchd3) gene is specifically expressed in male germ cells.
  • Ptchd3 protein is localized on the surface of sperm in mice, rats, and humans.
  • The presence of a conserved patched domain suggests a potential role as a membrane receptor for hedgehog ligands.

Purpose of the Study:

  • To investigate the function of Ptchd3 in mouse development and spermatogenesis.
  • To determine if Ptchd3 plays a role in the hedgehog signaling pathway.

Main Methods:

  • Generation and analysis of a Ptchd3 knockout mouse model.
  • Assessment of developmental and reproductive phenotypes in knockout mice.
  • Evaluation of gene expression levels in the testes of mutant mice.

Main Results:

  • Ptchd3 knockout mice exhibited normal development and survival.
  • Male knockout mice showed no alterations in fertility or sperm production.
  • Expression levels of key hedgehog signaling pathway genes remained unaffected in the mutant testes.

Conclusions:

  • Ptchd3 is not essential for normal mouse development.
  • Ptchd3 does not appear to play a critical role in spermatogenesis or male fertility.
  • The function of Ptchd3 in relation to hedgehog signaling in male germ cells requires further investigation.