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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Dec 18, 2025

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Published on: September 18, 2014

Using flow cytometry for mitochondrial assays.

Lauar de Brito Monteiro¹, Gustavo Gastão Davanzo¹, Cristhiane Favero de Aguiar¹

¹Division of Metabolism, Experimental Medicine Research Cluster (EMRC), and Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, SP, Brazil.

|June 20, 2020

Summary

This summary is machine-generated.

This study presents a flow cytometry protocol to assess immune cell mitochondrial health. It enables rapid evaluation of mitochondrial mass, membrane potential, and reactive oxygen species (ROS) production in live cells.

Keywords:

Immunometabolism Metabolic reprogramming Mitochondrial function Mitochondrial membrane potential

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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Published on: December 26, 2019

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Last Updated: Dec 18, 2025

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Published on: September 18, 2014

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Published on: December 26, 2019

Area of Science:

Immunology
Cellular Metabolism
Mitochondrial Biology

Background:

Immune cell metabolism significantly influences immune responses during pathogen invasion or tissue damage.
Activated immune cells typically shift from mitochondrial respiration to glycolysis for rapid ATP production.
This metabolic shift alters mitochondrial membrane potential and increases reactive oxygen species (ROS) generation.

Purpose of the Study:

To establish a flow cytometry-based protocol for evaluating mitochondrial fitness in immune cells.
To provide a simplified method for assessing mitochondrial parameters in live immune cells.

Main Methods:

Utilizing flow cytometry to track changes in mitochondrial mass, membrane potential, and superoxide (ROS) production.
Simultaneously employing MitoTracker Green and MitoTracker Red/MitoSOX probes for multi-parametric analysis.

Main Results:

Demonstrated the capability of flow cytometry to monitor mitochondrial dynamics in live immune cells.
Validated a protocol for quick evaluation of mitochondrial fitness using combined fluorescent probes.

Conclusions:

The proposed flow cytometry protocol offers a streamlined approach for assessing mitochondrial health in immune cells.
This method aids in understanding immune cell metabolism and facilitates high-throughput screening in immunology research.