Lipid Profiling and Microstructure Characteristics of Goat Milk Fat from Different Stages of Lactation

Goat milk at different lactations show varied lipids distributions, which are potentially dietary influencing factors for the health of human consumers. Herein, the effects of lactation stages (colostral, transitional, and mature stages) on lipid profiling and microstructure of goat milk fat (GMF) were investigated. A total of 359 species of triacylglycerols (TAGs), 27 species of diacylglycerols (DAGs), and 10 classes of phospholipids (PLs) were identified using high resolution tandem mass spectrometry (HR-MS/MS). Of importance, goat transitional milk presented the highest levels of MUFA (29.51%) and lyso-phospholipids (7.95% of total PLs) among these three different lactations. A lactation-dependent attenuation was found at the level of PUFA in goat milk, particularly long-chain PUFA ω-6. Similar behavior was observed in the total proportions of POO (16:0/18:1/18:1) and PSL (16:0/18:0/18:2), presenting a decrease from 3.70% to 3.23% as the proceeding period from colostrum to mature. The relative contents of sphingomyelin and cholesterol in goat colostrum were approximately twice and three times that in mature milk, respectively. Unlikely, both PMCy+MCaM (16:0/14:0/8:0 + 14:0/10:0/14:0) and BuPO (4:0/16:0/18:1) TAGs, the foremost saturated and monounsaturated TAGs in goat colostrum, respectively, showed upward trends over the period from colostrum to mature. Interestingly, no significant variation in milk fat globule morphology was monitored at different lactation periods. Therefore, all our results demonstrated that the main influences of lactation stages on GMF were the lipid profiling, providing a theoretical guidance for rational implement of lipids in goat milk.