Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance...

Area of Science:

• Biotechnology
• Virology
• Cancer Immunotherapy

Background:

• Oncolytic viruses are crucial for cancer immunotherapy, but their clinical application is hindered by inefficient purification methods.
• Current purification technologies limit the quantity, quality, and timeliness of oncolytic virus production for clinical use.
• Chromatography is a promising technology for large-scale virus purification, but existing methods face challenges with low yields and long processing times.

Purpose of the Study:

• To develop a fast, simple, and inexpensive downstream processing method for purifying oncolytic viruses.
• To improve the yield and purity of clinical-grade oncolytic Newcastle disease virus (NDV).
• To enable scalable and efficient production of oncolytic virotherapies.

Main Methods:

• Development of an interference chromatography process utilizing NatriFlo® HD-Q membrane technology.
• Optimization of chromatographic performance based on differences in molecular bonding interactions.
• Single ion exchange step for high-purity viral agent recovery.

Main Results:

• Successful purification of high-titer, clinical-grade oncolytic Newcastle disease virus.
• Achieved high purity in a single ion exchange step through interference chromatography.
• Demonstrated a high-yield purification method suitable for large-scale applications.

Conclusions:

• Interference chromatography combined with membrane technology offers an efficient and scalable purification solution for oncolytic virotherapies.
• This novel method has the potential to overcome current limitations in virus production for clinical use.
• Enables viable production of oncolytic virotherapies by improving process efficiency and yield.