Dickeya oryzae sp. nov., isolated from the roots of rice

A novel Gram-stain-negative strain, designated ZYY5^T, was isolated from rice roots. Results of 16S rRNA gene analysis indicated that strain ZYY5^T was a member of the genus Dickeya, with a highest similarity to Dickeya zeae DSM 18068^T (98.5%). The major fatty acids were summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), C_16:0 and summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c). Multi-locus sequence analysis using five concatenated genes (16S rRNA, atpD, infB, recA and gyrB) and phylogenomic analysis based on 2940 core gene sequences showed that strain ZYY5^T formed a robust cluster with strains EC1, ZJU1202, DZ2Q, NCPPB 3531 and CSL RW192, while separated from the other strains of D. zeae. The orthologous average nucleotide identity (ANI) and digital DNA-DNAhybridization (dDDH) values among these six strains ranged from 96.8-99.9% and 73.7-99.8%, which supported that they were belonged to the same species. However, strain ZYY5^T shared 58.4 of dDDH and 94.5% of ANI values with type strain D. zeae DSM 18068^T, which were lower than the proposed species boundary cut-off for dDDH and ANI. The genomic analysis revealed that strain ZYY5^T contained virulence-associated genes, which is same as the phylogenetic-related strains of the genus Dickeya. Based on the results of the polyphasic approaches, we propose that strain ZYY5^T represents a novel species in the genus Dickeya, for which the name Dickeya oryzae sp. nov. (=JCM 33020 ^T=ACCC 61554 ^T) is proposed. Strains EC1, ZJU1202, DZ2Q, NCPPB 3531 and CSL RW192 should also be classified in the same genomospecies of D. oryzae same as ZYY5^T.