Related Experiment Video

Updated: Dec 18, 2025

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

357

Communication between Cyclin-dependent kinase Cdc2 and the Wis1-Spc1 MAPK pathway determines mitotic timing in

Agamani Ghosal1, Priyanka Sarkar1, Geetanjali Sundaram2

  • 1Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, WB, India.

Biology Open
|June 20, 2020
PubMed

Insights

Mitotic cyclin-dependent kinase (CDK) activity influences stress-responsive mitogen-activated protein kinase (MAPK) activation in fission yeast. This CDK-MAPK interplay is crucial for regulating cell division timing.

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Yeast Genetics

Background:

  • Cellular survival under stress relies on checkpoint activation and gene expression modulation.
  • Cyclin-dependent kinases (CDKs) regulate cell cycle checkpoints, while mitogen-activated protein kinases (MAPKs) control gene expression.
  • The reciprocal regulation between cell cycle progression and MAPK-mediated stress response is not fully understood.

Purpose of the Study:

  • To investigate the influence of mitotic CDK activity on MAPK activation in *Schizosaccharomyces pombe*.
  • To explore the reciprocal relationship between cell-cycle regulation and stress response pathways.

Main Methods:

  • Genetic manipulation of mitotic CDK (Cdc2) activity in *S. pombe*.
  • Assay of stress-responsive MAPK (Spc1) activation levels.
  • Observation of MAPK activation in the absence of external stress stimuli.

Main Results:

  • Altering mitotic CDK (Cdc2) activity directly impacts the activation of the stress-responsive MAPK (Spc1) in *S. pombe*.
  • This effect on Spc1 MAPK activation occurs even without the presence of stress.
  • A significant correlation exists between Cdc2 activity and Spc1 MAPK activity, influencing mitotic timing.

Conclusions:

  • Mitotic CDK activity plays a regulatory role in the extent of MAPK activation in fission yeast.
  • The interplay between Cdc2 and Spc1 is essential for proper mitotic timing.
  • This study reveals a novel link between cell-cycle control and stress response pathways in yeast.
Keywords:
Cdc2MitosisRad24S. pombeSpc1Wis1

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