Streptomyces boluensis sp. nov., isolated from lake sediment

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537^T, was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537^T showed that it is closely related to the type strain of Streptomyces ziwulingensis F22^T (97.9% sequence similarity), Streptomyces tauricus JCM 4837 ^T (97.7%) and Streptomyces beijiangensis NBRC 100044 ^T (97.6%). The cell wall of the strain contained LL-diaminopimelic acid and the cell-wall sugars were glucose, galactose and ribose. The major phospholipids of strain YC537^T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H₆) and MK-9(H₈). The major cellular fatty acids were iso-C_16:0, iso-C_14:0, anteiso-C_15:0 and iso-C_15:0. Consequently, strain YC537^T is considered to represent a novel species in the genus Streptomyces, for which the name Streptomyces boluensis sp. nov. is proposed. The type strain is YC537^T (= KCTC 39750 ^T = DSM 102303 ^T).