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Tranexamic acid quantification in human whole blood using liquid samples or volumetric absorptive microsampling
Elodie Lamy1, Ileana Runge1, Ian Roberts2
1Département de Biotechnologie de la Santé, Université Paris-Saclay, UVSQ, INSERM, Infection et Inflammation, Montigny le Bretonneux, France.
Bioanalysis
|June 20, 2020
Summary
This study validates a sensitive method for measuring tranexamic acid in whole blood, utilizing microsampling devices for easier sample collection and analysis. This approach offers excellent performance and stability for pharmacokinetic investigations.
Area of Science:
- Pharmacology
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Tranexamic acid is an antifibrinolytic drug with demonstrated clinical benefits.
- In-depth pharmacokinetic studies of tranexamic acid are needed.
- Volumetric absorptive microsampling offers advantages over traditional plasma analysis for dried whole blood samples.
Purpose of the Study:
- To develop and validate high-sensitivity LC-MS/MS methods for quantifying tranexamic acid.
- To analyze tranexamic acid in both liquid and dried whole blood samples using microsampling devices.
- To assess the stability of tranexamic acid in stored blood samples.
Main Methods:
- Development and validation of high-sensitivity liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods.
- Quantification of tranexamic acid in human whole blood (liquid and dried samples).
- Validation according to international guidelines (IATDMCT, EMA, FDA).
Main Results:
- Excellent method performance across clinically relevant concentrations.
- Demonstrated stability of tranexamic acid in blood samples stored for up to 1 month at +50°C.
- Successful confirmation of method suitability using clinical samples.
Conclusions:
- Validated LC-MS/MS methods enable accurate tranexamic acid quantification in whole blood.
- Microsampling devices provide a viable and advantageous alternative for tranexamic acid pharmacokinetic studies.
- The developed methods are suitable for analyzing clinical samples and support further research into tranexamic acid pharmacokinetics.
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