Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.4K
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

130.9K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
130.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.5K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

15.9K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
15.9K
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

3.6K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isosteric Engineering of Enzymes: Overcoming Activity-Stability Trade-Offs by Site-Selective CH → N Substitutions.

Angewandte Chemie (International ed. in English)·2026
Same author

Artificial allosteric protein switches with machine-learning-designed receptors.

Nature biotechnology·2026
Same author

Classification of uterine sarcoma and leiomyoma with quantitative CT and dual-energy iodine mapping.

Clinical imaging·2026
Same author

Advances in polarised luminescence approaches to understanding interactions between biomolecules.

Biochemical Society transactions·2026
Same author

Biosynthetic Lanthanide-Luminescent Mini-Proteins Using Genetic Code Expansion.

Journal of the American Chemical Society·2026
Same author

Iso-pseudoprolines as versatile tools for late-stage peptide backbone modifications.

Chemical science·2026

Related Experiment Video

Updated: Dec 18, 2025

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

27.7K

Playing Tag with Your Favorite GPCR Using CRISPR.

Mizuho Horioka1, Thomas Huber2, Thomas P Sakmar2

  • 1Tri-Institutional Program in Chemical Biology, The Rockefeller University, 1230 York Ave., New York, NY, USA; Laboratory of Chemical Biology and Signal Transduction, The Rockefeller University, 1230 York Ave., New York, NY, USA.

Cell Chemical Biology
|June 20, 2020
PubMed
Summary

Researchers developed a new method using CRISPR/Cas9 to tag G protein-coupled receptors (GPCRs) and β-arrestin. This technique allows for studying receptor signaling under natural genetic conditions, opening new research avenues.

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.6K
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.9K

Related Experiment Videos

Last Updated: Dec 18, 2025

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

27.7K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.6K
Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

8.9K

Area of Science:

  • Cellular biology
  • Molecular biology
  • Biochemistry

Background:

  • G protein-coupled receptors (GPCRs) are crucial cell surface receptors involved in numerous physiological processes.
  • Understanding GPCR signaling pathways is essential for developing targeted therapeutics.
  • Existing methods for studying GPCRs often rely on overexpression systems, which may not reflect endogenous signaling accurately.

Purpose of the Study:

  • To develop a novel method for studying GPCR and β-arrestin signaling.
  • To enable the investigation of receptor signaling under endogenous genetic control.
  • To provide new tools for the analysis of GPCR function.

Main Methods:

  • CRISPR/Cas9 gene editing was employed to tag GPCRs and β-arrestin within their endogenous genomic loci.
  • Luminescence reporter and complementation technologies were integrated with the CRISPR/Cas9 tagging strategy.
  • The developed method was applied to study receptor signaling in cellular models.

Main Results:

  • Successful CRISPR/Cas9-mediated tagging of GPCRs and β-arrestin was achieved.
  • The method allows for the study of receptor signaling under endogenous genetic conditions.
  • The integration with luminescence technologies provides a sensitive readout of signaling events.

Conclusions:

  • CRISPR/Cas9-mediated tagging offers a powerful approach to study GPCR and β-arrestin signaling.
  • This method facilitates the investigation of receptor function in a physiologically relevant context.
  • The developed strategy opens new avenues for exploring GPCR biology and drug discovery.