Highly effective fibrin biopolymer scaffold for stem cells upgrading bone regeneration
Camila Fernanda Zorzella Creste1,2, Patrícia Rodrigues Orsi1, Fernanda Cruz Landim-Alvarenga3
1Center for the Study of Venoms and Venomous Animals (CEVAP), UNESP-São Paulo State University, Botucatu 18610-307, Brazil.
Abstract:
Fibrin scaffold fits as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. We evaluated a unique heterologous fibrin biopolymer as scaffold to mesenchymal stem cells (MSCs) to treat a critical-size bone defect. Femurs of 27 rats were treated with fibrin biopolymer (FBP); FBP + MSCs; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was evaluated 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP + MSC-D association was the most effective treatment, since newly formed Bone was more abundant and early matured in just 21 days. We concluded that FBP is an excellent scaffold for MSCs and also use of differentiated cells should be encouraged in regenerative therapy researches. The FBP ability to maintain viable MSCs at Bone defect site has modified inflammatory environment and accelerating their regeneration.
