Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.3K
3.3K
RNA Splicing01:32

RNA Splicing

60.0K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.0K
Types of RNA01:20

Types of RNA

8.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
8.6K
Types of RNA01:23

Types of RNA

72.1K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.1K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.4K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urinary Small Extracellular Vesicle DNA as a Biomarker for the Non-Invasive Diagnosis of Bladder Cancer.

Cancer science·2026
Same author

The WHO Classification of Genetic Tumour Syndromes: Considerations for Genetics.

Clinical genetics·2026
Same author

System for study of diverse cellular composition in alveolar rhabdomyosarcoma.

Scientific reports·2026
Same author

LPAR4 mediates resistance to interferon-induced stress in soft tissue sarcoma.

Science advances·2026
Same author

Pan-cancer spatial atlas of tertiary lymphoid structures.

Science (New York, N.Y.)·2026
Same author

MAFF regulates ferroptotic sensitivity through iron homeostasis and fatty acid synthesis.

Cell death & disease·2026

Related Experiment Video

Updated: Dec 17, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.0K

The Long Noncoding RNA NEAT1 Promotes Sarcoma Metastasis by Regulating RNA Splicing Pathways.

Jianguo Huang1, Mohit Sachdeva1, Eric Xu1

  • 1Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina.

Molecular Cancer Research : MCR
|June 21, 2020
PubMed
Summary
This summary is machine-generated.

The long noncoding RNA Nuclear Enriched Abundant Transcript 1 (Neat1) drives soft-tissue sarcoma metastasis by interacting with RNA splicing regulators like KHSRP, offering potential new therapeutic targets.

More Related Videos

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.7K

Related Experiment Videos

Last Updated: Dec 17, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.0K
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.7K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Soft-tissue sarcomas (STS) are rare cancers with high rates of lung metastasis.
  • Understanding the molecular drivers of STS metastasis is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the role of long noncoding RNAs (lncRNAs) in mediating STS lung metastasis.
  • To identify molecular mechanisms by which lncRNAs promote sarcoma progression.

Main Methods:

  • Utilized a genetically engineered mouse model (KP) mimicking undifferentiated pleomorphic sarcoma (UPS).
  • Employed RNA-sequencing to compare primary tumors and lung metastases.
  • Performed CRISPR/Cas9-mediated knockout and RNA immunoprecipitation followed by mass spectrometry.

Main Results:

  • The lncRNA Nuclear Enriched Abundant Transcript 1 (Neat1) was significantly upregulated in lung metastases of both mouse models and human UPS.
  • Neat1 knockout suppressed lung colonization in the mouse model.
  • Neat1 interacts with RNA splicing regulatory proteins, notably KH-Type Splicing Regulatory Protein (KHSRP), which is linked to poor prognosis.

Conclusions:

  • Neat1 plays a critical role in promoting soft-tissue sarcoma lung metastasis.
  • The interaction between Neat1 and KHSRP in regulating RNA splicing represents a potential therapeutic vulnerability in STS.