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CALIPER Hematology Reference Standards (I).

Houman Tahmasebi¹, Victoria Higgins^1,2, Mary Kathryn Bohn^1,2

¹CALIPER Program, Pediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Canada.

American Journal of Clinical Pathology

|June 21, 2020

Summary

This summary is machine-generated.

This study establishes crucial age- and sex-specific hematologic reference standards for 47 parameters in healthy children and adolescents. These findings highlight the need for pediatric-specific reference values for accurate clinical interpretation.

Keywords:

Beckman Coulter CALIPER Hematology Pediatric Reference intervals

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Area of Science:

Clinical Hematology
Pediatric Reference Intervals
Biostatistics

Background:

Accurate interpretation of hematologic tests relies on normative reference standards for clinical decision-making.
A significant lack of healthy pediatric reference data exists for most hematology parameters.
The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) cohort provides a unique dataset of healthy children and adolescents.

Purpose of the Study:

To establish age- and sex-specific hematologic reference standards for the pediatric population.
To address the critical gap in normative data for pediatric hematology parameters.
To provide a robust database for improved clinical interpretation of hematologic tests in children and adolescents.

Main Methods:

Analysis of 47 hematologic parameters from 566 healthy children and adolescents (birth to <21 years).
Utilized fresh whole blood samples analyzed on the Beckman Coulter DxH 900.
Calculated age- and sex-specific reference standards following Clinical and Laboratory Standards Institute guidelines.

Main Results:

Dynamic changes in reference value distributions were observed across the pediatric age range.
Significant age-specific differences were found for 39 of the 47 hematologic parameters.
Sex-specific differences were noted for eight parameters, particularly around puberty.

Conclusions:

Established the first robust database of pediatric reference standards for 47 hematologic parameters in the CALIPER cohort.
Reported physiologically relevant trends in hematologic markers, emphasizing pediatric-specific needs.
Demonstrated a clear need for dedicated pediatric reference standards for accurate hematologic test interpretation.