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Updated: Dec 17, 2025

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Collection and Identification of Pollen from Honey Bee Colonies
Published on: January 19, 2021
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Pollen Load Spectrum of Tomato Pollinators.
A P Hautequestt1, M S Deprá2, V Gonçalves-Esteves3
1Centro de Biociências e Biotecnologia, Lab de Ciências Ambientais, Pós-Graduação em Ecologia e Recursos Naturais, Campos dos Goytacazes, Univ Estadual do Norte Fluminense Darcy Ribeiro, Rio de Janeiro, Brasil. anna.hautequestt@gmail.com.
Neotropical Entomology
|June 21, 2020
Summary
Vibrating bees, crucial for tomato pollination, utilize diverse floral resources beyond tomatoes. Maintaining varied flowering plants near farms supports these essential pollinators.
Area of Science:
- Ecology
- Entomology
- Agricultural Science
Background:
- Vibrating bees are key pollinators for tomato (Solanum lycopersicum L.) crops.
- Understanding alternative food sources for bees is vital for effective pollinator management in agriculture.
Purpose of the Study:
- To identify food resources utilized by Bombus morio and Exomalopsis analis, major pollinators of tomatoes.
- To assess the dietary overlap and niche differentiation between these two bee species.
Main Methods:
- Pollen analysis from the hind legs of 40 Bombus morio and 72 Exomalopsis analis females.
- Comparison of collected pollen with a reference collection of 155 flowering plant species.
- Pollen identification using acetolysis and optical microscopy.
Main Results:
- Bombus morio and Exomalopsis analis collected pollen from numerous plant species, including tomato.
- Fabaceae, Hyptis, and Solanum species were significant non-tomato pollen sources.
- Trophic niche overlap was minimal when tomato pollen was excluded, indicating distinct foraging preferences.
Conclusions:
- Tomato pollinators exhibit generalist feeding behavior, utilizing diverse floral resources.
- The presence of alternative floral resources, even during tomato flowering, is crucial.
- Conservation of diverse flowering plants around agricultural areas is essential for pollinator support.

