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Refined Murine Model of Idiopathic Pulmonary Fibrosis

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Leonurus sibiricus root extracts decrease airway remodeling markers expression in fibroblasts.

J Wieczfinska¹, P Sitarek², T Kowalczyk³

¹Department of Immunopathology, Medical University of Lodz, Lodz, Poland.

Clinical and Experimental Immunology

|June 21, 2020

Summary

Transformed Leonurus sibiricus root extract, particularly with AtPAP1 overexpression, shows potential in combating airway remodeling in asthma. This extract reduced key inflammatory markers, suggesting a role for plant-derived compounds in lung disease treatment.

Keywords:

Lamiaceae Leonurus sibiricus TR and AtPAP1 TR plant extracts Western blot airway remodeling asthma gene expression rhinovirus 16 (HRV16)

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Area of Science:

Biotechnology
Pharmacology
Plant Science

Background:

Bronchial asthma involves airway inflammation and remodeling, a complex process crucial for disease progression.
Controlling airway remodeling is essential for managing asthma and other obstructive lung diseases.
Plants produce secondary metabolites with potential therapeutic properties, including anti-inflammatory and anti-remodeling effects.

Purpose of the Study:

To evaluate the anti-remodeling properties of extracts from transformed and transgenic Leonurus sibiricus roots.
Specifically, to assess the effects of an extract overexpressing the transcriptional factor AtPAP1 (AtPAP1 TR) on fibroblast cell lines.
To investigate the impact of these extracts on markers of airway remodeling under normal and rhinovirus-induced conditions.

Main Methods:

Two human fibroblast cell lines (WI-38 and HFL1) were treated with transformed L. sibiricus root extract (TR) and AtPAP1 TR extract.
Airway remodeling conditions were induced using rhinovirus 16 (HRV16).
Expression levels of metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), arginase I, and transforming growth factor-beta (TGF-β) were quantified using qPCR and immunoblotting.

Main Results:

AtPAP1 TR extract significantly decreased the expression of arginase I and MMP-9.
No significant effect was observed on TIMP-1 or TGF-β mRNA expression by the AtPAP1 TR extract alone.
The AtPAP1 TR extract inhibited HRV16-induced expression of arginase I, MMP-9, and TGF-β in both cell lines (P < 0.05).

Conclusions:

Transformed L. sibiricus root extract, particularly AtPAP1 TR, demonstrates anti-remodeling properties relevant to asthma.
The observed effects may be attributed to increased phenolic acids like chlorogenic acid, caffeic acid, and ferulic acid.
Biotechnology offers a valuable approach for discovering and developing plant-derived medicinal compounds for respiratory diseases.