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Related Experiment Video

Updated: Dec 17, 2025

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Performance analysis of high-throughput HPV testing on three automated workflows.

Anne J M Loonen1,2, Cornelis J J Huijsmans1, Willemina R R Geurts-Giele3

  • 1Pathologie-DNA, Lab for Molecular Diagnostics, Location Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|June 21, 2020
PubMed
Summary

The Hologic Aptima workflow demonstrated superior performance in high-risk human papillomavirus (hrHPV) DNA testing compared to Roche Cobas and Qiagen Digene HC2, offering optimal efficiency for laboratories. This evaluation of automated hrHPV testing workflows highlights key operational factors for selection.

Keywords:
AutomationhrHPV testingperformance analysis

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Area of Science:

  • Clinical diagnostics
  • Molecular biology
  • Public health

Background:

  • Primary high-risk human papillomavirus (hrHPV) DNA testing is increasingly adopted globally.
  • Standardized automated workflows are crucial for efficient and reliable hrHPV screening.

Purpose of the Study:

  • To compare the performance of three automated workflow procedures for hrHPV testing.
  • To evaluate operational aspects of hrHPV detection assays meeting international guidelines.

Main Methods:

  • A comparative study processed 15,000 residual cervical samples over three months.
  • Three automated platforms were evaluated: Qiagen Digene® HC2 HPV DNA test (HC2), Roche Cobas® HPV test, and Hologic Aptima® HPV test.
  • Key performance indicators included time-to-results, hands-on-time (HOT), processing capacity, and error rates.

Main Results:

  • The Hologic Aptima workflow achieved the highest overall score (7.2), followed by Roche Cobas (7.1), and Qiagen Digene HC2 (5.8).
  • Maximum processing capacities and HOT varied: Aptima (296 samples, 2h 55m), Cobas (282 samples, 3h 20m), and HC2 (264 samples, 4h 15m).
  • Throughput times per run were 6h 30m for 60 samples (Aptima) and 5h 51m for 94 samples (Cobas); HC2 results were available on day 2.

Conclusions:

  • While all tested hrHPV assays meet international guidelines, their workflow performance characteristics differ significantly.
  • The Hologic Aptima workflow is recommended for high-throughput hrHPV testing due to its superior operational efficiency.
  • Laboratory selection should consider hands-on-time, time-to-results, throughput, and other operational factors beyond assay performance.