Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.6K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.6K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.0K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Epigenetic Regulation01:37

Epigenetic Regulation

3.6K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.6K
Epigenetic Regulation01:46

Epigenetic Regulation

33.2K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HyperTRIBE identifies hepatic IGF2BP2/IMP2 targets <i>in vivo</i> and links IMP2 to autophagy.

NAR molecular medicine·2026
Same author

IGF2BP2 Deficiency in Macrophages Impairs Migration, Reprograms Metabolism, and Limits Tumor Progression.

International journal of biological sciences·2026
Same author

Hetero-oligomerization drives structural plasticity of eukaryotic peroxiredoxins.

Nature chemical biology·2026
Same author

Utilizing Molecular Docking to Investigate Some Phenolic Acid Phytochemical Interactions with Platelet Aggregation Pathway Proteins.

Pakistan journal of biological sciences : PJBS·2026
Same author

Alternatively spliced STIM2.3 is an evolutionarily late store-operated Ca2+ entry regulator expressed in brain.

Journal of cell science·2026
Same author

RNA-binding proteins connect Exon usage to the chromatin.

NAR genomics and bioinformatics·2025

Related Experiment Video

Updated: Dec 17, 2025

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

6.9K

DNA methylation and the core pluripotency network.

Siba Shanak1, Volkhard Helms2

  • 1Faculty of Science, Arab-American University, Jenin, Palestine; Center for Bioinformatics, Saarland University, Saarbruecken, Germany.

Developmental Biology
|June 21, 2020
PubMed
Summary

This review explores the dynamic relationship between DNA methyltransferases and core pluripotency factors like OCT4, SOX2, and NANOG. Understanding this interplay is key to controlling stem cell self-renewal and differentiation.

Keywords:
DNA methylationDifferentiationStem cellsTranscription factor

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.1K
Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.2K

Related Experiment Videos

Last Updated: Dec 17, 2025

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

6.9K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.1K
Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.2K

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Stem Cell Biology

Background:

  • Cellular development involves genome-wide changes, including epigenetic modifications.
  • Stem cell pluripotency and differentiation are regulated by complex molecular networks.
  • The core pluripotency network includes key transcription factors OCT4, SOX2, and NANOG.

Purpose of the Study:

  • To elucidate the intricate interplay between DNA methyltransferases and core pluripotency factors.
  • To highlight how DNA methylation regulates pluripotency factor expression.
  • To examine how pluripotency factors control DNA methyltransferase transcription.

Main Methods:

  • Review of existing literature on gene regulation and epigenetics.
  • Analysis of DNA methylation dynamics during cell fate transitions.
  • Integration of structural studies and computer simulations for atomistic detail.

Main Results:

  • DNA methyltransferases modulate DNA methylation in enhancer regions of core pluripotency factors.
  • Core pluripotency factors regulate the transcription of DNA methyltransferases.
  • A reciprocal regulatory loop exists between DNA methylation and pluripotency factors.

Conclusions:

  • The interplay between DNA methylation and pluripotency factors is crucial for stem cell fate.
  • Understanding this epigenetic crosstalk offers insights into developmental processes.
  • This review provides a comprehensive overview of this regulatory network.