Preparation of galactose oxidase functional phosphorescent quantum dots and detection of D-galactose

Environmental friendly nano biosensor can improve the detection performance of traditional biomolecular sensors and have important application value in practical applications. In this study, a kind of room temperature phosphorescence (RTP) quantum dots (QDs) (GOX RTP QDs) nanobiosensor was prepared by mineralization at room temperature (25 °C), using galactose oxidase (GOX) as template, which improved the catalytic ability of traditional GOx to D-Galactose. The specific enzyme substrate reaction between GOx and D-Galactose and photoinduced electron transfer (Piet) were used to detect the RTP of D-galactose. The linear range of D-galactose detection is 0.02-0.8 mM, and the detection limit of the method is 0.008 mM. This method is based on the RTP property of QDs, which can effectively avoid the interference of background fluorescence of biological samples, and does not need complex sample pretreatment process. Therefore, this method is more suitable for the quantitative detection of D-Galactose in biological samples.