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RBC membrane instability for large pipette deformation. A theoretical approach
M M Kozlov1, D Lerche, W Meier
1Dept. Haemorheology and Haemodynamics, Humboldt-University, Berlin, German Democratic Republic.
Biorheology
|January 1, 1988
Summary
Micropipette aspiration experiments reveal a "pinch-off" phenomenon. Theoretical analysis identified conditions for membrane instability, dependent on pressure differences and membrane tension, providing insights into vesicle size.
Area of Science:
- Biophysics
- Cell Mechanics
- Fluid Dynamics
Background:
- Micropipette aspiration experiments have previously observed a
- pinch-off
Purpose of the Study:
- To theoretically analyze the conditions leading to membrane instability during micropipette aspiration.
- To investigate the factors influencing the
- pinch-off
Main Methods:
- Theoretical analysis of effective transmembrane pressure difference.
- Approximation of hydrodynamic flow through the gap between cell tongue and pipette.
- Consideration of membrane tension and cell tongue length.
Main Results:
- Identified a condition for membrane instability when the membrane tongue exceeds 3 times the pipette radius (3Rp).
- Instability occurs at a point of minimum effective transmembrane pressure drop.
- Derived an expression for the condition of instability and vesicle size.
Conclusions:
- The study provides a theoretical framework for understanding membrane instability and pinch-off.
- Effective transmembrane pressure, influenced by flow and membrane tension, is critical.
- The findings offer insights into the mechanics of cell membrane deformation and rupture.