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Published on: June 13, 2014
The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass
Priscila da Silva Delabona1, Carla Aloia Codima2, Jonas Ramoni3
1National Centre of Research in Energy and Materials, High-tech Pole II, 10000 Giuseppe Maximo Scolfaro St, P.O Box 6192, Campinas, SP, Brazil; Synthetic Biology and Molecular Biotechnology, Research Division Biochemical Technology, Institute of Chemical Engineering, TU Wien, Gumpendorferstrasse 1a, A-1060 Vienna, Austria; Federal University of Goiás (UFG), Samambaia Campus, Goiânia, GO, Brazil.
Abstract:
Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1(20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum.
