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Apical extrusion of intracanal biofilm using ProTaper Gold, WaveOne Gold,Twisted File Adaptive, OneShape New
Recai Zan1, Tutku Tunç2, İhsan Hubbezoğlu3
1Department of Endodontics, Cumhuriyet University School of Dentistry, Sivas, Turkey.
Objective:
To evaluate the bacterial extrusion during instrumentation with different nickel titanium (NiTi) engine-driven instruments.
Methods:
Ninety extracted single-canal human mandibular incisor teeth were inoculated with Enterococcus faecalis to obtain biofilm formation and were randomly divided to 6 groups (n=15). One group served as the control and was not instrumented; the other groups were prepared with ProTaper Gold (PTG; Dentsply Maillefer, Ballaigues, Switzerland), WaveOne Gold (WOG; Dentsply Maillefer), Twisted File Adaptive (TFA; SybronEndo, Orange, CA, USA), One Shape New Generation (OSNG; MicroMega, Besancon, France), and K3XF (SybronEndo) instruments. Bacteria extruded beyond the apical foramen were quantified in colony-forming units per milliliter. The number of colony-forming units in the remaining biofilm was determined for each sample. Data were analyzed using the one-way analysis of variance (ANOVA) and Tukey post-hoc tests.
Results:
All NiTi instruments resulted in different quantities of bacterial extrusion. The TFA group caused most bacterial extrusion (P<0.05). The PTG and WOG groups caused less bacterial extrusion than the OSNG and K3XF groups (P<0.05), but there was no statistically significant difference between the PTG and WOG groups (P>0.05).
Conclusion:
PTG and WOG are preferable system in terms of successful endodontic treatments. The amount of bacterial extrusion is associated with the metallurgy and design of the instrument used.
