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Published on: May 24, 2014
The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia
Kouji Sugimoto1, Yoshihiko Hayashi2
1Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Abstract:
The characteristics of pluripotent cells have great potential for basic and clinical research and application. We describe the effect of normoxia or hypoxia regarding the proliferation and pluripotency of human iPS cells using colony number counting and real-time polymerase chain reaction (PCR). In addition, the function of hypoxia-inducible factors (HIFs) in human iPS cells under hypoxic conditions is evaluated in relation to the expression of pluripotency markers by siRNA and real-time PCR. Furthermore, we introduce the change of HIF-2α expression when signal transducer and activator of transcription 3 (STAT3) is suppressed by its inhibitor, Stattic or S31 201, using RT-PCR.
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