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A novel method for nucleic acid sequence determination.

W Bains¹, G C Smith

¹Department of Biochemistry, University of Bath, U.K.

Journal of Theoretical Biology

|December 7, 1988

Summary

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A new automated sequencing method fragments DNA and quantifies pieces using hybridization. This allows for reconstruction of the original sequence from fragment data, offering a practical approach to DNA sequencing.

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Current DNA sequencing technologies face challenges in automation and cost-effectiveness.
Accurate reconstruction of nucleotide sequences is crucial for various biological applications.

Purpose of the Study:

To introduce a novel, fully automatable sequencing methodology.
To present a practical protocol for implementing this new sequencing approach.

Main Methods:

The method involves fragmenting nucleotide or deoxynucleotide sequences into short segments.
Quantitation of these fragments is achieved through hybridization to specific oligo-deoxynucleotides immobilized on a solid support.
The original sequence is reconstructed computationally from the frequency data of the identified fragments.

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Main Results:

Demonstration of a novel sequencing methodology with potential for complete automation.
Presentation of a specific protocol enabling practical implementation of the described approach.
The method allows for sequence reconstruction based on fragment frequency analysis.

Conclusions:

The described sequencing methodology offers a promising avenue for automated and efficient DNA sequencing.
The presented protocol facilitates the practical application of this innovative technique in molecular biology and genomics.