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Related Experiment Videos

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains.

Tino Emanuele Poloni1, Valentina Medici2, Arenn Faye Carlos3

  • 1Department of Neurology and Neuropathology, Golgi-Cenci Foundation; Department of Rehabilitation, ASP Golgi-Redaelli Geriatric Hospital; e.poloni@golgicenci.it.

Journal of Visualized Experiments : Jove
|June 23, 2020
PubMed
Summary

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This study introduces a novel brain banking protocol for high-quality tissue analysis. It enables comprehensive neuropathological and omics studies for understanding neurodegenerative diseases.

Area of Science:

  • Neuroscience
  • Pathology
  • Biotechnology

Background:

  • Aging populations are increasing the prevalence of neurodegenerative disorders.
  • Understanding disease mechanisms requires direct examination of brain tissue.
  • Existing methods may not fully capture the complexity of asymmetrical neurological diseases.

Purpose of the Study:

  • To present a protocol for obtaining, processing, characterizing, and storing high-quality brain tissue.
  • To establish a comprehensive brain bank for neurodegenerative disease research.
  • To facilitate neuropathologically verified epidemiological studies.

Main Methods:

  • Implementing an antemortem brain donation program with empathic engagement.
  • Collecting extensive clinical, biological, social, and lifestyle data.

Related Experiment Videos

  • Utilizing a unique protocol for slicing fresh brain specimens, alternating between freezing and formalin fixation for each hemisphere.

    • Main Results:

    • The protocol yields good quality brain tissue suitable for detailed analysis.
    • The alternating fixation/freezing method allows for complete histological characterization of frozen tissues.
    • Omics studies can be performed on histologically defined tissues from both hemispheres, offering a more complete assessment.

    Conclusions:

    • This brain banking protocol provides a comprehensive approach to studying neurodegenerative disease mechanisms.
    • Combining clinical data with neuropathological evaluation is crucial for accurate diagnosis and understanding pathogenesis.
    • The established protocol supports the development of the first Italian Brain Bank and advances neuropathologically verified epidemiological research.