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Updated: Dec 17, 2025

Preparation and Characterization of Lipophilic Doxorubicin Pro-drug Micelles
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Doxorubicin-loading core-shell pectin nanocell: A novel nanovehicle for anticancer agent delivery with multidrug

Jiabi Ouyang1, Mohui Yang1, Tian Gong1

  • 1College of Pharmacy, Jinan University, Guangzhou, China.

Plos One
|June 23, 2020
PubMed

Insights

This study introduces a novel doxorubicin-loaded pectin nanocell (DOX-PEC-NC) to combat multidrug resistance (MDR) in cancer. The nanocell enhances drug delivery, reverses MDR, and improves anticancer efficacy with reduced toxicity.

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Oncology

Background:

  • Multidrug resistance (MDR) in tumors is a major obstacle to successful chemotherapy.
  • Doxorubicin (DOX), a widely used anticancer drug, often faces efficacy limitations due to MDR.
  • Nanomedicine offers potential solutions for overcoming drug resistance in cancer treatment.

Purpose of the Study:

  • To develop and characterize a novel pectin nanocell encapsulating doxorubicin (DOX-PEC-NC) for improved cancer therapy.
  • To evaluate the drug release profile, in vitro anticancer activity, and in vivo efficacy of DOX-PEC-NC.
  • To investigate the potential of DOX-PEC-NC in reversing multidrug resistance (MDR) in cancer cells.

Main Methods:

  • Fabrication of a core-shell structured pectin nanocell (DOX-PEC-NC) encapsulating doxorubicin.
  • Assessment of sustained drug release behavior compared to conventional doxorubicin formulations.
  • In vitro evaluation of cellular uptake and reversal of MDR in resistant cancer cell lines (HepG2/ADR, MCF-7/ADR).
  • In vivo efficacy and toxicity studies in H22 tumor-bearing mice.

Main Results:

  • DOX-PEC-NC exhibited a sustained release profile superior to doxorubicin-loaded pectin nanoparticles (DOX-PEC-NP) and liposomes (DOX-LIP).
  • In vitro studies demonstrated enhanced intracellular accumulation of doxorubicin and significant reversal of MDR in HepG2/ADR and MCF-7/ADR cells.
  • In vivo studies in H22 tumor-bearing mice showed that DOX-PEC-NC possessed higher anticancer efficacy and lower toxicity compared to free doxorubicin.

Conclusions:

  • DOX-PEC-NC effectively enhances the anticancer activity of doxorubicin by increasing intracellular drug accumulation and reversing MDR.
  • The developed nanocell formulation demonstrates potential as a promising nanoscale drug delivery vehicle for chemotherapeutic agents.
  • DOX-PEC-NC offers a strategy to improve therapeutic outcomes and reduce side effects associated with doxorubicin chemotherapy.

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