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Multiple Non-coding ANRIL Transcripts Are Associated with Risk of Coronary Artery Disease: a Promising Circulating

Juan Fang1, Zhicheng Pan1, Dongfei Wang1

  • 1Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China.

Journal of Cardiovascular Translational Research
|June 24, 2020
PubMed

Insights

This study investigated ANRIL isoforms in coronary artery disease (CAD). Circulating circANRIL(exon14-4) showed potential as a biomarker for diagnosing CAD and predicting major adverse cardiovascular events (MACE).

Area of Science:

  • Molecular Biology
  • Cardiovascular Disease Research
  • Biomarker Discovery

Background:

  • ANRIL is a long non-coding RNA with multiple transcriptional isoforms.
  • Dysregulation of ANRIL isoforms is implicated in various diseases, including cardiovascular conditions.
  • The diagnostic and prognostic utility of specific ANRIL isoforms in coronary artery disease (CAD) requires further investigation.

Purpose of the Study:

  • To explore the diagnostic value of six ANRIL transcriptional isoforms in patients with coronary artery disease (CAD).
  • To identify the most promising ANRIL isoform as a circulating biomarker for CAD.
  • To evaluate the prognostic value of the identified biomarker in relation to clinical outcomes and major adverse cardiovascular events (MACE).

Main Methods:

  • Selection and measurement of six target ANRIL isoforms' expression in peripheral blood of CAD patients and controls.
  • Evaluation of diagnostic performance of individual ANRIL isoforms.
  • Validation of the diagnostic and prognostic value of the best-performing isoform in a larger clinical cohort.
  • Correlation analysis between circANRIL(exon14-4) expression levels and MACE incidence.

Main Results:

  • lncANRIL(exon1) and lncANRIL(exon4-6) were significantly increased in CAD patients, while circANRIL(exon14-4) was downregulated.
  • circANRIL(exon14-4) demonstrated the highest diagnostic value among the evaluated isoforms.
  • Lower expression of circANRIL(exon14-4) was significantly associated with an increased incidence of MACE.
  • Combination of circANRIL(exon14-4) with other factors improved CAD patient differentiation.

Conclusions:

  • circANRIL(exon14-4) exhibits significant diagnostic value for coronary artery disease.
  • Downregulation of circANRIL(exon14-4) is linked to increased risk and severity of CAD.
  • circANRIL(exon14-4) represents a promising circulating biomarker for both the diagnosis and prognosis of CAD.

Keywords:
BiomarkerCoronary artery diseasecircANRILlncANRIL

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