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Linc00703 suppresses non-small cell lung cancer progression by modulating CyclinD1/CDK4 expression

H-B Sun1, X-L Han, M Zhong

  • 1Department of Thoracic Surgery, The Third Hospital of Jilin University, Changchun, China. yudujuan@jlu.edu.cn.

European Review for Medical and Pharmacological Sciences
|June 24, 2020
PubMed
Abstract

Insights

Long non-coding RNA linc00703 is downregulated in non-small cell lung cancer (NSCLC). Overexpressing linc00703 inhibits NSCLC cell proliferation and promotes apoptosis by affecting cyclinD1/CDK4 expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality worldwide.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.
  • The specific function of linc00703 in NSCLC remains largely unexplored.

Purpose of the Study:

  • To investigate the expression levels of linc00703 in NSCLC tissues and cells.
  • To elucidate the biological functions of linc00703 in NSCLC proliferation, apoptosis, and cell cycle.
  • To explore the potential molecular mechanism underlying linc00703's role in NSCLC.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect linc00703 expression in NSCLC tissues and cell lines.
  • In vitro experiments involved transfecting NSCLC cells with linc00703 overexpression plasmids.
  • Cell proliferation, apoptosis, cell cycle distribution, and downstream protein expression were assessed using CCK-8, colony formation, flow cytometry, and Western blotting.

Main Results:

  • linc00703 expression was significantly downregulated in NSCLC tissues and cells compared to normal controls.
  • Overexpression of linc00703 inhibited NSCLC cell proliferation and induced apoptosis.
  • linc00703 overexpression led to G1/G0 phase arrest and decreased protein levels of cyclinD1 and CDK4.

Conclusions:

  • linc00703 acts as a tumor suppressor in NSCLC.
  • The findings suggest that linc00703 suppresses NSCLC progression by downregulating cyclinD1/CDK4 expression.
  • linc00703 may represent a potential therapeutic target for NSCLC.

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