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MiRNA-1246 suppresses the proliferation and migration of renal cell carcinoma through targeting CXCR4

H-T Liu1, W-X Fan

  • 1Department of Urology Surgery, Daqing Oilfield General Hospital, Daqing, China. fanwx1999@163.com.

European Review for Medical and Pharmacological Sciences
|June 24, 2020
PubMed
Abstract

Insights

MicroRNA-1246 (miRNA-1246) is downregulated in renal cell carcinoma (RCC), suppressing tumor growth and migration by targeting CXCR4. This miRNA-1246/CXCR4 interaction presents a potential therapeutic strategy for RCC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Renal cell carcinoma (RCC) is a significant global health concern.
  • Understanding the molecular mechanisms driving RCC proliferation and metastasis is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of microRNA-1246 (miRNA-1246) in regulating the proliferative and migratory capacities of RCC.
  • To elucidate the interaction between miRNA-1246 and CXCR4 in RCC pathogenesis.

Main Methods:

  • Quantitative analysis of miRNA-1246 and CXCR4 expression in 40 paired RCC and normal tissues.
  • Dual-Luciferase reporter assay to confirm the binding relationship between miRNA-1246 and CXCR4.
  • Assessment of RCC cell proliferation and migration following miRNA-1246 or CXCR4 manipulation.

Main Results:

  • MiRNA-1246 was found to be significantly downregulated in RCC tissues and cell lines.
  • Overexpression of miRNA-1246 inhibited the proliferation and migration of RCC cells (786-O and 769-P).
  • CXCR4 was identified as a direct target of miRNA-1246, with its expression negatively regulated by miRNA-1246. Silencing CXCR4 also inhibited RCC proliferation and migration.

Conclusions:

  • MiRNA-1246 suppresses RCC proliferation and migration by downregulating CXCR4.
  • The miRNA-1246/CXCR4 axis represents a promising therapeutic target for renal cell carcinoma.

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