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MicroRNA-133a-5p inhibiting metastatic capacity of renal clear cell carcinoma through regulating MON2

P Li1, Q Wu, X-R Yang

  • 1Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang, China. suntingdr@163.com.

European Review for Medical and Pharmacological Sciences
|June 24, 2020
PubMed
Abstract

Insights

Reduced microRNA-133a-5p expression in clear cell renal cell carcinoma (ccRCC) predicts poor prognosis. This microRNA suppresses ccRCC cell proliferation and metastasis by targeting MON2.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Clear cell renal cell carcinoma (ccRCC) is a significant health concern.
  • Understanding the molecular mechanisms underlying ccRCC progression is crucial for developing effective therapies.
  • MicroRNAs play vital roles in cancer development and progression.

Purpose of the Study:

  • To investigate the correlation between microRNA-133a-5p expression and clinicopathological parameters in ccRCC patients.
  • To explore the functional role of microRNA-133a-5p in ccRCC cell biological behavior.
  • To elucidate the underlying molecular mechanism of microRNA-133a-5p in ccRCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to examine microRNA-133a-5p and MON2 expression in ccRCC tissues and cell lines.
  • Cell Counting Kit-8 (CCK-8) and transwell assays were performed to evaluate the effects of microRNA-133a-5p on ccRCC cell proliferation and invasion.
  • Bioinformatics analysis and Luciferase assays were employed to identify and validate the downstream target of microRNA-133a-5p.

Main Results:

  • MicroRNA-133a-5p expression was significantly reduced in ccRCC tissues and cell lines, correlating with poor prognosis.
  • Overexpression of microRNA-133a-5p inhibited the proliferation and migration of ccRCC cells.
  • MON2 was identified as a direct target of microRNA-133a-5p, and its expression was inversely correlated with microRNA-133a-5p in ccRCC.

Conclusions:

  • Reduced microRNA-133a-5p expression serves as a potential biomarker for predicting poor prognosis in ccRCC patients.
  • MicroRNA-133a-5p suppresses ccRCC cell proliferation and metastasis.
  • The inhibitory effects of microRNA-133a-5p on ccRCC progression are mediated through its regulation of MON2.

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