Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Yield Production and Cost-Effective Purification of an Alkali-Resistant Hexameric Protein A Variant for Antibody Affinity Chromatography.

Biotechnology journal·2026
Same author

One-Pot Synthesis of a Small Synthetic Ligand for Antibody Purification.

Biotechnology and bioengineering·2026
Same author

Tailoring the Properties of Ethyl Cellulose Membranes Using Hydrophobic Deep Eutectic Solvents.

ACS sustainable chemistry & engineering·2026
Same author

Multi-omics characterization of developing forebrain organoids unravels the dynamic molecular events of Rett syndrome pathogenesis.

Journal of neurodevelopmental disorders·2026
Same author

Discovery and optimization of a synthetic small protein domain targeting antibodies.

Frontiers in bioengineering and biotechnology·2025
Same author

The goat Longissimus thoracis et lumborum muscle proteome: effects of dry ageing and cooking in chevon.

Food research international (Ottawa, Ont.)·2025

Related Experiment Video

Updated: Dec 17, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.6K

Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and

Cláudia S M Fernandes1, André L Rodrigues2, Vitor D Alves3

  • 1UCIBIO, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal.

Biomacromolecules
|June 24, 2020
PubMed
Summary

Researchers developed novel affinity-triggered hydrogels for cell encapsulation using the strong avidin-biotin interaction. These biocompatible hydrogels successfully supported stem cell differentiation, offering a tunable platform for tissue engineering.

More Related Videos

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.8K
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

7.5K

Related Experiment Videos

Last Updated: Dec 17, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
15:33

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

29.6K
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.8K
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

7.5K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Stem Cell Biology

Background:

  • Hydrogels are crucial for cell encapsulation, supporting cell expansion and differentiation in tissue engineering.
  • Affinity-triggered hydrogels offer mild cell encapsulation via selective ligand-target interactions and multivalency.
  • Designing hydrogel properties based on affinity and multivalency remains a significant challenge.

Purpose of the Study:

  • To investigate the influence of multivalency on hydrogel properties using a high-affinity pair.
  • To develop robust, tunable, and biocompatible synthetic protein hydrogels for cell encapsulation.
  • To demonstrate the utility of these hydrogels for induced pluripotent stem cell encapsulation and neural differentiation.

Main Methods:

  • Utilized the avidin-biotin affinity pair for hydrogel formation.
  • Investigated the impact of multivalent display on hydrogel binding and stability.
  • Assessed hydrogel biocompatibility and performance in encapsulating and differentiating induced pluripotent stem cells.

Main Results:

  • Hydrogel binding is significantly influenced by the multivalent display of affinity components.
  • Achieved robust synthetic protein hydrogels by adhering to natural multivalency principles.
  • Demonstrated stoichiometric matching is critical for hydrogel resistance to erosion.
  • Confirmed hydrogel biocompatibility and successful differentiation of encapsulated stem cells into neural lineages.

Conclusions:

  • Affinity-triggered hydrogels can be designed with controlled properties by leveraging specific affinity pairs and multivalency.
  • This approach provides a versatile platform for creating dynamic biomaterials for regenerative medicine.
  • The principle is generalizable to other affinity pairs and multimeric proteins for advanced biomaterial development.