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MicroRNA-4331-5p promotes FMDV replication through inhibiting interferon pathways in PK-15 cells

Tingting Ren1, Yanxue Wang2, Haotai Chen1

  • 1State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.

Virus Research
|June 24, 2020
PubMed

Insights

MicroRNA ssc-miR-4331-5p enhances foot-and-mouth disease virus (FMDV) replication by suppressing type I interferon pathways. This microRNA (miRNA) plays a key role in the host

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • MicroRNAs (miRNAs) are crucial regulators in host-pathogen interactions.
  • Previous studies linked miR-4331 to Transmissible Gastroenteritis Virus and Influenza A virus.
  • The role of ssc-miR-4331-5p in Foot-and-Mouth Disease Virus (FMDV) infection was not established.

Purpose of the Study:

  • To investigate the role of ssc-miR-4331-5p in FMDV replication.
  • To determine the regulatory mechanism of ssc-miR-4331-5p during FMDV infection.

Main Methods:

  • Detection of ssc-miR-4331-5p expression in porcine kidney (PK-15) cells post-FMDV infection.
  • Transfection of miR-4331-5p mimics and inhibitors to assess effects on FMDV replication.
  • Analysis of type I interferon pathway activation.

Main Results:

  • FMDV infection significantly upregulated ssc-miR-4331-5p expression in PK-15 cells.
  • Overexpression of miR-4331-5p promoted FMDV replication, while inhibition suppressed it.
  • ssc-miR-4331-5p was found to increase FMDV replication by inhibiting type I interferon signaling.

Conclusions:

  • ssc-miR-4331-5p plays a significant role in promoting FMDV replication.
  • The mechanism involves the suppression of type I interferon pathways.
  • This miRNA represents a potential target for antiviral strategies against FMDV.
Keywords:
FMDVInterferonmiRNAs

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