Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying best practices for RTTs and dosimetrists: a Delphi consensus study on standardizing workflows in proton therapy.

Technical innovations & patient support in radiation oncology·2026
Same author

Improved deconvolution of sequenced based mixtures by extending EuroForMix.

Forensic science international. Genetics·2026
Same author

Internal validation of the fully continuous model in EuroForMix for its implementation in routine forensic DNA profiling.

Forensic science international. Genetics·2026
Same author

Towards proton therapy guidelines for radiation therapists and dosimetrists: A scoping review.

Technical innovations & patient support in radiation oncology·2025
Same author

A pilot study on alternative blood sampling methods in pigs.

BMC veterinary research·2025
Same author

Evaluating the effect of marker panel sizes on estimation of bio-geographical co-ancestry proportions.

Forensic science international. Genetics·2025

Related Experiment Video

Updated: Dec 17, 2025

Holistic Facial Composite Creation and Subsequent Video Line-up Eyewitness Identification Paradigm
09:49

Holistic Facial Composite Creation and Subsequent Video Line-up Eyewitness Identification Paradigm

Published on: December 24, 2015

14.5K

Development and optimization of the VISAGE basic prototype tool for forensic age estimation.

A Heidegger1, C Xavier1, H Niederstätter1

  • 1Institute of Legal Medicine, Medical University of Innsbruck, Innsbruck, Austria.

Forensic Science International. Genetics
|June 24, 2020
PubMed
Summary
This summary is machine-generated.

The VISAGE consortium developed a DNA methylation tool for forensic age estimation. This prototype reliably quantifies methylation levels at 32 CpG sites, aiding forensic science applications.

Keywords:
Age estimationBisulfite PCR multiplex developmentMiSeq FGx sequencingTargeted bisulfite sequencing

More Related Videos

Author Spotlight: Development of an Automated Camera-Based System for Real-Time Blast Overpressure Monitoring and TBI Risk Assessment in Military Training
06:20

Author Spotlight: Development of an Automated Camera-Based System for Real-Time Blast Overpressure Monitoring and TBI Risk Assessment in Military Training

Published on: December 6, 2024

3.1K
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

4.8K

Related Experiment Videos

Last Updated: Dec 17, 2025

Holistic Facial Composite Creation and Subsequent Video Line-up Eyewitness Identification Paradigm
09:49

Holistic Facial Composite Creation and Subsequent Video Line-up Eyewitness Identification Paradigm

Published on: December 24, 2015

14.5K
Author Spotlight: Development of an Automated Camera-Based System for Real-Time Blast Overpressure Monitoring and TBI Risk Assessment in Military Training
06:20

Author Spotlight: Development of an Automated Camera-Based System for Real-Time Blast Overpressure Monitoring and TBI Risk Assessment in Military Training

Published on: December 6, 2024

3.1K
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

4.8K

Area of Science:

  • Forensic science
  • Genomics
  • Epigenetics

Background:

  • DNA intelligence methods are increasingly used in forensic science.
  • Epigenetic modifications, specifically DNA methylation, offer potential for forensic age estimation.

Purpose of the Study:

  • To develop and validate a prototype tool for forensic age estimation using DNA methylation.
  • To optimize and assess the performance of the VISAGE basic prototype assay.

Main Methods:

  • Targeting 32 CpG sites across five genes (ELOVL2, MIR29B2C, FHL2, TRIM59, KLF14).
  • Utilizing multiplex PCR on bisulfite-converted DNA followed by massively parallel sequencing (MiSeq FGx).
  • Optimizing bisulfite conversion protocols and evaluating assay reproducibility and sensitivity.

Main Results:

  • Robust quantification of DNA methylation levels with a mean standard deviation of 1.4%.
  • The assay demonstrated sensitivity down to 20 ng DNA input without increased variability.
  • Median difference between technical replicates was below 1.6%.

Conclusions:

  • The VISAGE basic prototype tool shows promise for reliable forensic age estimation.
  • Optimized protocols ensure assay robustness and sensitivity for routine laboratory use.
  • This tool broadens the application of DNA intelligence in forensic investigations.