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Updated: Dec 17, 2025
Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
Published on: August 17, 2022
Preimplantation Genetic Testing: Where We Are Today
Ermanno Greco1,2, Katarzyna Litwicka1, Maria Giulia Minasi1
1Reproductive Medicine, Villa Mafalda, 00199 Rome, Italy.
Background:
Preimplantation genetic testing (PGT) is widely used today in in-vitro fertilization (IVF) centers over the world for selecting euploid embryos for transfer and to improve clinical outcomes in terms of embryo implantation, clinical pregnancy, and live birth rates.
Methods:
We report the current knowledge concerning these procedures and the results from different clinical indications in which PGT is commonly applied.
Results:
This paper illustrates different molecular techniques used for this purpose and the clinical significance of the different oocyte and embryo stage (polar bodies, cleavage embryo, and blastocyst) at which it is possible to perform sampling biopsies for PGT. Finally, genetic origin and clinical significance of embryo mosaicism are illustrated.
Conclusions:
The preimplantation genetic testing is a valid technique to evaluated embryo euploidy and mosaicism before transfer.
