Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent advances in nanomaterial-based biosensors for testosterone detection.

The Analyst·2025
Same author

Neurocognitive Outcomes After Extracranial Surgery and General Anesthesia in Patients with a History of Mild-to-Moderate Traumatic Brain Injury: Systemic Review and Meta-Analysis.

Biology·2025
Same author

Comparison of Wound Healing Effects of Different Micro-Patterned Hydrogels on the Skin of Secondary Intention Rat Model.

Gels (Basel, Switzerland)·2025
Same author

Perioperative Neurocognitive Disorder in Individuals with a History of Traumatic Brain Injury: Protocol for a Systematic Review and Meta-Analysis.

Biology·2025
Same author

Comparative Efficacy of Glucosamine-Based Combination Therapies in Alleviating Knee Osteoarthritis Pain: A Systematic Review and Network Meta-Analysis.

Journal of clinical medicine·2024
Same author

On-Site Bioaerosol Sampling and Airborne Microorganism Detection Technologies.

Biosensors·2024

Related Experiment Video

Updated: Jun 19, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.3K

Label-Free Electrochemical Microfluidic Chip for the Antimicrobial Susceptibility Testing.

Hyoil Jeon1, Zeeshan A Khan1, Emad Barakat1

  • 1School of Mechanical Engineering, Korea University of Technology and Education, Cheonan, Chungnam 31253, Korea.

Antibiotics (Basel, Switzerland)
|June 25, 2020
PubMed
Summary

This study introduces an automated electrochemical microfluidic chip (EMC) for rapid antibiotic susceptibility testing (AST). The EMC significantly reduces testing time compared to conventional methods, aiding in timely treatment and combating antibiotic resistance.

Keywords:
ASTE. coliUTIslabel-freemicrofluid chip

More Related Videos

Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.8K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.7K

Related Experiment Videos

Last Updated: Jun 19, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.3K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.8K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.7K

Area of Science:

  • Biomedical Engineering
  • Clinical Diagnostics
  • Microfluidics

Background:

  • Antibiotic resistance is a critical global health threat.
  • Current antibiotic susceptibility testing (AST) methods are often slow, complex, and costly.
  • There is a need for rapid, automated, and user-friendly AST solutions.

Purpose of the Study:

  • To develop an automated electrochemical microfluidic chip (EMC) for simple and rapid AST.
  • To enable faster bacterial count and minimum inhibitory concentration (MIC) determination.
  • To reduce the time and resources required for AST.

Main Methods:

  • Design and fabrication of a microfluidic chip with integrated gold microelectrodes.
  • Automation of antibiotic mixing and distribution within microfluidic chambers.
  • Electrical signal measurements for bacterial detection and AST.
  • Comparison with conventional broth microdilution and AlamarBlue assays.

Main Results:

  • The EMC achieved rapid bacterial count in 170 minutes and AST in 150 minutes.
  • Testing time was significantly reduced compared to conventional broth microdilution (450 min for count, 240 min for AST).
  • Accurate MIC evaluation was demonstrated on the EMC, comparable to established methods.
  • The chip showed sensitivity, minimal antibiotic loss, and potential for reducing resistance evolution.

Conclusions:

  • An automated, label-free, economical, and rapid EMC for AST has been successfully developed.
  • The EMC offers a user-friendly and robust platform for evaluating AST, particularly in urine samples.
  • This technology has the potential to improve patient treatment and control the spread of antibiotic resistance.