Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.5K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.5K
Histone Modification02:32

Histone Modification

15.6K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.6K
Histone Modification02:32

Histone Modification

4.2K
4.2K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

8.4K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody-trapping presents a widespread pitfall for microscopy and genomics in the nucleus.

Nucleic acids research·2026
Same author

Marine Pharmacology in 2022-2023: Marine Compounds with Antibacterial, Antidiabetic, Antifungal, Anti-Inflammatory, Antiprotozoal, Antituberculosis and Antiviral Activities, Affecting the Immune and Nervous Systems, and Other Miscellaneous Mechanisms of Action.

Marine drugs·2026
Same author

Importin α characterizes a micronuclear environment associated with genomic instability in human cancer cells.

Journal of cell science·2026
Same author

NELF prevents transcriptional readthrough into DNA replication zones in cancer cells.

EMBO reports·2026
Same author

AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications.

Science advances·2026
Same author

Reconstructing epigenomic dynamics through a single-cell multi-epigenome data integration framework.

Nature communications·2025

Related Experiment Video

Updated: Dec 17, 2025

Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.4K

Histone modification dynamics as revealed by multicolor immunofluorescence-based single-cell analysis.

Yoko Hayashi-Takanaka1,2, Yuto Kina3, Fumiaki Nakamura3

  • 1Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan ythayashi@fbs.osaka-u.ac.jp hkimura@bio.titech.ac.jp.

Journal of Cell Science
|June 25, 2020
PubMed
Summary

This study introduces a multicolor immunofluorescence method to track histone modifications in single cells. The technique reveals dynamic changes in epigenetic marks during the cell cycle and aids in identifying compounds affecting these modifications.

Keywords:
Chemical biologyChromatinEpigeneticsHistone modificationMonoclonal antibody

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.1K
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

18.8K

Related Experiment Videos

Last Updated: Dec 17, 2025

Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.4K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

10.1K
Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

18.8K

Area of Science:

  • * Epigenetics and Molecular Biology
  • * Cell Biology
  • * Chemical Biology

Background:

  • * Histone modifications are crucial epigenetic marks influencing gene expression.
  • * These modifications dynamically change during the cell cycle and in response to stimuli.
  • * Current methods for analyzing histone modifications can be complex and time-consuming.

Purpose of the Study:

  • * To develop a simple, multicolor immunofluorescence system for monitoring multiple histone modifications in single cells.
  • * To analyze dynamic changes in histone modifications during the cell cycle.
  • * To validate histone demethylase targets and screen for compounds affecting histone modifications.

Main Methods:

  • * Multicolor immunofluorescence using directly labeled, modification-specific antibodies.
  • * Single-cell analysis of histone H3 and H4 modifications.
  • * Transient overexpression systems for target validation.
  • * Screening of chemical compounds from marine organism extracts.

Main Results:

  • * Identified cell cycle-dependent fluctuations in active (acetylation, H3K4 methylation) and repressive histone marks.
  • * Active marks increased during S phase, associated with chromatin duplication.
  • * Repressive marks gradually increased during G2 and G1 phases.
  • * Validated histone demethylase targets and identified psammaplin A as a histone deacetylase inhibitor.

Conclusions:

  • * The developed multicolor immunofluorescence method is a powerful and convenient tool for analyzing histone modification dynamics.
  • * This system facilitates the study of epigenetic regulation during the cell cycle.
  • * The method aids in the discovery and validation of compounds targeting histone-modifying enzymes.