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Updated: Dec 17, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
Published on: April 14, 2015
Sensing cellular biochemistry with fluorescent chemical-genetic hybrids
Arnaud Gautier1, Alison G Tebo2
1Sorbonne Université, École Normale Supérieure, PSL University, CNRS, Laboratoire des Biomolécules, LBM, 75005 Paris, France; Institut Universitaire de France, France.
Abstract:
Fluorescent biosensors are powerful tools for the detection of biochemical events inside cells with high spatiotemporal resolution. Biosensors based on fluorescent proteins often suffer from issues with photostability and brightness. On the other hand, hybrid, chemical-genetic systems present unique opportunities to combine the strengths of synthetic, organic chemistry with biological macromolecules to generate exquisitely tailored semisynthetic sensors.
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