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Dynamically Expressed miR-BART16 Functions as a Suppressor of CAND1 in Infectious Mononucleosis Caused by

Zhenhua Hu1, Xiuxiu Zheng2, Wanjie Zeng1

  • 1Department of Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medicine, Suzhou, China.

Annals of Clinical and Laboratory Science
|June 26, 2020
PubMed
Abstract

Insights

Epstein-Barr Virus-encoded miR-BART16 (EBV-miR-BART16) is elevated in children with infectious mononucleosis (IM). This microRNA regulates CAND1 expression, impacting pediatric IM.

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Epstein-Barr Virus (EBV) is a common human herpesvirus.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression.
  • EBV encodes its own miRNAs, including EBV-miR-BART16.

Purpose of the Study:

  • To investigate the role of EBV-encoded miR-BART16 in pediatric infectious mononucleosis (IM).
  • To determine if EBV-miR-BART16 levels correlate with IM.
  • To identify targets of EBV-miR-BART16 in the context of EBV infection.

Main Methods:

  • Analysis of peripheral blood lymphocyte subsets in IM patients and healthy children using flow cytometry.
  • Quantification of EBV-miR-BART16 and its potential targets via real-time PCR, western blot, ELISA, and dual-luciferase assays.
  • Receiver operating characteristic (ROC) analysis to assess diagnostic potential.

Main Results:

  • Serum EBV-miR-BART16 levels were significantly higher in children with IM compared to healthy controls.
  • Serum EBV-miR-BART16 expression positively correlated with EBV viral load.
  • ROC analysis indicated serum EBV-miR-BART16 can distinguish IM patients from healthy individuals (P=0.0041).
  • CAND1 was identified as a direct target of EBV-miR-BART16, with its expression downregulated in an EBV-dependent manner.

Conclusions:

  • EBV-miR-BART16 plays a significant role in the pathogenesis of pediatric infectious mononucleosis.
  • The study highlights EBV-miR-BART16's function in regulating CAND1 expression.
  • EBV-miR-BART16 serves as a potential biomarker for diagnosing pediatric IM.
Keywords:
CAND1Epstein-Barr virusInfectious mononucleosismiRNA

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