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Single-atom Ni-N4 provides a robust cellular NO sensor.

Min Zhou1,2, Ying Jiang3, Guo Wang1

  • 1Department of Chemistry, Capital Normal University, Beijing, 100048, China.

Nature Communications
|June 26, 2020
PubMed
Summary
This summary is machine-generated.

We developed a novel electrochemical sensor using single-atom catalysts (SACs) for real-time nitric oxide (NO) detection in cells. This Ni SACs/N-C sensor offers high sensitivity and biocompatibility for cellular studies.

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Nitric oxide (NO) plays crucial roles in physiological and pathological processes.
  • Accurate monitoring of cellular NO levels requires sensitive, biocompatible sensors with transient recording capabilities.
  • Existing sensors often lack the necessary performance for real-time cellular NO detection.

Purpose of the Study:

  • To develop a novel single-atom catalyst (SAC)-based electrochemical sensor for detecting nitric oxide (NO) in live cellular environments.
  • To evaluate the sensor's performance, including sensitivity, biocompatibility, and transient recording ability.
  • To demonstrate the sensor's utility in real-time NO monitoring under cellular stimulation.

Main Methods:

  • Fabrication of a sensor using nickel single atoms anchored on N-doped hollow carbon spheres (Ni SACs/N-C).
  • Electrochemical characterization of Ni SACs/N-C for NO oxidation catalysis.
  • Testing the sensor's performance in live cellular environments, including response to drug and stretch stimulation.
  • Assessing sensor biocompatibility and sensitivity at the nanomolar level.

Main Results:

  • Ni SACs/N-C exhibited superior electrocatalytic performance for NO oxidation compared to conventional Ni nanomaterials due to reduced activation energy.
  • The developed sensor demonstrated high biocompatibility and low nanomolar sensitivity.
  • Real-time monitoring of NO release from cells was successfully achieved upon drug and stretch stimulation.
  • The flexible and stretchable nature of the sensor enhanced its applicability in dynamic cellular conditions.

Conclusions:

  • Single-atom catalysts (SACs) provide a promising platform for developing highly efficient electrochemical sensors.
  • The Ni SACs/N-C sensor enables sensitive and real-time detection of NO in live cells.
  • This technology offers a valuable tool for investigating NO-related biological processes and cellular responses.