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Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass

Laurent Dortet1,2,3,4, Rémy A Bonnin2,3,4, Simon Le Hello5

  • 1MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United Kingdom.

Frontiers in Microbiology
|June 26, 2020
PubMed

Insights

Detecting colistin resistance in Gram-negative bacteria is crucial. The MALDIxin test offers a rapid method for identifying colistin-resistant Salmonella enterica, improving upon traditional time-consuming techniques.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Mass Spectrometry

Background:

  • Polymyxin resistance in Gram-negative bacteria is primarily due to modifications in the lipopolysaccharide (LPS) lipid A component.
  • Resistance mechanisms involve chromosomal mutations or plasmid-encoded mcr genes, increasing the complexity of colistin resistance.
  • Conventional minimal inhibitory concentration (MIC) determination via broth microdilution is time-consuming (24 hours) and difficult for routine laboratories.

Purpose of the Study:

  • To introduce and evaluate the MALDIxin test as a rapid method for detecting colistin resistance in Salmonella enterica.
  • To assess the feasibility of using the MALDIxin test on a standard matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.

Main Methods:

  • Utilized the MALDIxin test for the detection of colistin resistance.
  • Employed the matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system for analysis.
  • Focused on Salmonella enterica as the target organism.

Main Results:

  • Demonstrated the successful application of the MALDIxin test for identifying colistin-resistant Salmonella enterica.
  • Showcased the MALDIxin test's compatibility with the routine MALDI Biotyper Sirius platform.

Conclusions:

  • The MALDIxin test provides a rapid and effective alternative for detecting colistin resistance in Salmonella enterica.
  • This method streamlines the identification process compared to traditional MIC determination, aiding clinical and veterinary diagnostics.
Keywords:
MALDI mass spectrometrySalmonella entericacolistindiagno sticlipid A

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