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Rodent Leukocyte Isolation and Radiolabeling for Inflammation Imaging Study
Eun-Mi Kim1, Phil-Sun Oh1, Fatima Boud1
1Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk Republic of Korea.
Nuclear Medicine and Molecular Imaging
|June 26, 2020
Summary
This study developed methods for radiolabeling leukocytes in rats for inflammation imaging. Radiolabeled leukocytes successfully visualized inflamed lesions, offering insights into inflammatory diseases.
Area of Science:
- Biomedical imaging
- Radiochemistry
- Immunology
Background:
- In vivo imaging of inflammation is crucial for diagnosing and monitoring various diseases.
- Developing reliable methods for leukocyte isolation and radiolabeling is essential for accurate inflammation detection.
Purpose of the Study:
- To establish and optimize rodent leukocyte isolation and radiolabeling techniques.
- To evaluate the efficacy of radiolabeled leukocytes for in vivo inflammation imaging in rats.
Main Methods:
- Leukocyte isolation from rat blood using Histopaque density gradient centrifugation.
- Radiolabeling of isolated leukocytes with Technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO) and Fluorine-18 fluorodeoxyglucose (18F-FDG).
- In vivo imaging of induced thigh muscle inflammation in rats using radiolabeled leukocytes.
Main Results:
- Histopaque separation yielded a lymphocyte-rich leukocyte population.
- CO2 incubation maintained leukocyte viability, crucial for successful radiolabeling.
- 99mTc-HMPAO and 18F-FDG labeling efficiencies were 4.00±2.06% and 1.8%, respectively.
- Both radiolabeled leukocytes effectively targeted inflamed lesions, with 99mTc-HMPAO showing abdominal distribution.
Conclusions:
- Autologous radiolabeled leukocytes effectively visualized inflamed lesions in rats.
- The developed method provides a valuable tool for studying inflammatory diseases in vivo.
