A New Highly Selective Colorimetric and Fluorometric Coumarin-based Chemosensor for Hg²

A novel colorimetric and fluorometric method based on coumarin as signalling unit was developed for Hg²⁺ recognition and quantification. Initially, the alkyne functionality was incorporated into a coumarin system and the resulting molecule showed higher specificity and sensitivity for Hg²⁺ over other cations in both absorption and emission sensing assays. The Hg²⁺ recognition was detected as visible colour change from colourless to yellow and as fluorescence quenching. The colour change was assigned to the increased intramolecular charge transfer (ICT) in the signalling unit upon Hg²⁺ binding whereas a decline in the fluorescence intensity was ascribed to the heavy atom effect from Hg²⁺. In order to generate a material with high sensing performance level, alkyne-functionalized molecule was hosted into a polymeric material. The resulting functionalized polymer showed higher sensitivity and selectivity for Hg²⁺ over its corresponding coumarin molecule. The investigation of the possible binding modes for Hg²⁺ suggested both alkyne and triazole functionalities as potential binding sites for Hg²⁺. The limit of detection (LOD) and limit of quantification (LOQ) of the proposed method were evaluated and values less than a recommended maximum level of Hg²⁺contaminant in drinking water (2.00 μg/L) were obtained (LOD = 0.44 μg/L and LOQ = 1.33μg/L). The real-life application of the method was investigated using natural water samples containing Hg²⁺ levels equivalent to the maximum tolerable concentration of Hg²⁺ in drinking water. The outcomes suggested that the method could be used in the sensing and determination of Hg²⁺ level of contaminant in the environment.