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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

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LINC01426 contributes to clear cell renal cell carcinoma progression by modulating CTBP1/miR-423-5p/FOXM1 axis via

YuFeng Jiang1, HaiMin Zhang2, Wei Li2

  • 1Department of Urology, Chongming Branch, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

Journal of Cellular Physiology
|June 26, 2020
PubMed

Insights

Long intergenic nonprotein coding RNA 1426 (LINC01426) promotes clear cell renal cell carcinoma (ccRCC) progression by regulating the IGF2BP1/CTBP1/HDAC2/miR-423-5p/FOXM1 axis. This study identifies LINC01426 as a potential therapeutic target for ccRCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Long noncoding RNAs (lncRNAs) play critical roles in oncogenesis.
  • The specific functions of many lncRNAs in clear cell renal cell carcinoma (ccRCC) remain largely unknown.
  • The Cancer Genome Atlas data suggests LINC01426 is highly expressed in ccRCC and linked to poor prognosis.

Purpose of the Study:

  • To investigate the role and mechanism of long intergenic nonprotein coding RNA 1426 (LINC01426) in ccRCC.
  • To elucidate the regulatory axis involving LINC01426, miR-423-5p, and FOXM1 in ccRCC.
  • To identify LINC01426 as a potential therapeutic target for ccRCC.

Main Methods:

  • Analysis of The Cancer Genome Atlas data for LINC01426 expression in ccRCC.
  • Experimental validation of LINC01426 upregulation in ccRCC cell lines.
  • Assessment of LINC01426 depletion effects on ccRCC cell proliferation and migration.
  • Investigation of the interaction between LINC01426, miR-423-5p, FOXM1, CTBP1, HDAC2, and IGF2BP1 using molecular biology techniques.

Main Results:

  • LINC01426 is upregulated in ccRCC and its depletion inhibits cell proliferation and migration.
  • LINC01426 promotes ccRCC tumorigenesis by regulating the FOXM1 pathway.
  • LINC01426 positively regulates FOXM1 by inhibiting miR-423-5p, which is transcriptionally silenced by CTBP1 and HDAC2.
  • LINC01426 interacts with IGF2BP1 and CTBP1 to modulate miR-423-5p expression in both cytoplasm and nucleus, ultimately promoting ccRCC progression.

Conclusions:

  • LINC01426 exacerbates ccRCC progression through the IGF2BP1/CTBP1/HDAC2/miR-423-5p/FOXM1 axis.
  • LINC01426 represents a novel and promising therapeutic target for ccRCC.
Keywords:
CTBP1FOXM1IGF2BP1LINC01426ccRCCmiR-423-5p

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