Related Experiment Video
Updated: Jul 24, 2026
An Experimental System to Study Mechanotransduction in Fetal Lung Cells
Published on: February 16, 2012
In vivo Raman spectroscopy study on the stimulation mechanism of surfactant
Background:
Surfactant is widely used in skin care products and cleansers, while it may cause physical discomfort. In this study, in vivo Raman spectroscopy was used to explore surfactant irritation mechanism on skin, which was not found in literature.
Methods:
Sodium dodecyl sulfate (SDS) was chosen to represent surfactant. Research on the negative effect of SDS was undertaken by scanning the two states of the skin (without and with the contact of SDS), respectively, on six volunteers, by means of Raman technique and skin magnifier.
Results:
The damage to the surface of normal skin by SDS was visible from the photographs taken by skin magnifier, and the apparent damage matched the damage that was happening underneath the skin elucidated by Raman spectra. Compared to the normal skin, the inter-cellular lipids (ICL) lateral packing order of the damaged skin was significantly reduced in 2-12 μm of skin depth (P < 0.05), deeper than 12 μm was not detected. The skin depth of 0-2 μm could not be determined due to strong interference of SDS. Significant change in the secondary and tertiary structures of keratin was not found.
