Physicochemical properties of dentinogenesis imperfecta with a known DSPP mutation
Haemin Park1, Hong-Keun Hyun1, Kyung Mi Woo2
1Department of Pediatric Dentistry, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
Archives of Oral Biology
|June 26, 2020
Summary
A 1-base pair deletion in the dentin sialophosphoprotein (DSPP) gene affects tooth properties. This genetic change results in reduced dentin mineral density, microhardness, and magnesium content.
Area of Science:
- Dental science
- Genetics
- Materials science
Background:
- Dentin sialophosphoprotein (DSPP) is crucial for dentin formation and mineralization.
- Genetic mutations in DSPP can lead to dental anomalies, impacting tooth structure and properties.
Purpose of the Study:
- To investigate the chemical and mechanical properties of dentin affected by a specific 1-base pair deletion (c.2688delT) in the DSPP gene.
- To characterize the structural and compositional changes in teeth with this DSPP mutation.
Main Methods:
- Analysis of maxillary first premolars from a 9-year-old individual with the DSPP mutation.
- Utilized micro-computerized tomography (μCT), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), and Vickers microhardness testing.
Main Results:
- μCT revealed an obliterated pulp chamber and reduced pulpal volume in the affected sample.
- Dentin from the affected sample showed lower mineral density, microhardness, and magnesium (Mg) content compared to wild-type.
- Irregular obliteration of the pulp chamber was observed.
Conclusions:
- A 1-bp deletion in the DSPP gene significantly alters dentin properties.
- Affected dentin exhibits reduced mineralization, diminished mechanical strength, and lower Mg content.
- These findings highlight the critical role of DSPP in maintaining dentin integrity.
